B3.3

Purpose of the course:

The purpose of the course is to introduce the student to chromatographic methods of analysis, which are one of the most important methods of analysis used in artworks. Their only disadvantage is the requirement to take a small sample of the artwork, which is destroyed. However, this is compensated for by the absence of interference, the accuracy and sensitivity of the analysis and the certainty of the identity of the substance being analysed. Translated with www.DeepL.com/Translator (free version)

Educational objectives:

The aim of the course is first to provide and understand the theoretical background of chromatographic methods and then to specialize in specific methods including gas chromatography, liquid chromatography and combined techniques such as gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS).

The educational targets of this subject is the understanding of the theoretical background of chromatographic methods and mass spectroscopy and the knowledge of their use for the identification and quantitative determination of various compounds. Also, some commonly used techniques for the analysis of solutes in various phases of mixture samples, like Gas Chromatography (GC) and High-Pressure Liquid Chromatography (HPLC) as well as their combination with mass spectrometry (GC-MS and LC-MS) are studied.

Analysis of the lesson:

Modules and content of lectures by teacher:

1. 1. Georgios Kyriacou (Associate Professor, Dpt. of Chemical Engineering, AUTH).

Theoretical background of chromatography, qualitative and quantitative determination of compounds as well as gas chromatography with packed and capillary columns. Application of chromatographic methods for the analysis of components of artifacts.

2. Roxani Tzimou-Tsitouridou (Professor, Dpt. of Chemical Engineering; AUTH).

High Pressure Liquid Chromatography HPLC), Performance of detectors, columns, pumps and laboratory demonstration of a HPLC instrument.

3. Antigoni Kotali (Professor, Dpt. of Chemical Engineering, AUTH).

Scope of teaching of her subject is the understanding of mass spectra and their interpretation in the identification of compounds. Also, the understanding the performance of combination of Mass Spectra with Gas Chromatography(GC-MS) and with Liquid Chromatography (LC-MS).

4. Ioannis Karapanagiotis (Professor, Chemistry AUTH)

His subject is the training of students in the combined technique of Liquid Chromatography and Mass Spectroscopy (LC-MS). The scope of the laboratory exercise is the training of students in the analysis of organic dyestuffs.

Work or exercise

The instructors give bibliographical assignments mainly related to the course, but they are not compulsory for the students.

Evaluation

Examination (80%), exercise (20%)